Samuel Spencer was born 27 July 1644 in East Haddam, Middlesex County, Connecticut to Gerrard Spencer (1614-1685) and Hannah Hills (1618-1692) . He married Hannah Willey (1642-1681) 1 March 1673 in Hartford County, Connecticut.
Children
| Name | Birth | Death | Joined with |
| Grace Spencer (1674-) | |||
| John Spencer (1676-1749) | 17 September 1676 East Haddam, Middlesex County, Connecticut | 10 November 1749 East Haddam, Middlesex County, Connecticut | Elisabeth Brooks (1680-1725) Ruth Watrous (1687-) |
| Isaac Spencer (1678-1751) | |||
Siblings
| Name | Birth | Death | Joined with |
| John Spencer (1636-1682) | |||
| Mehitable Spencer (1638-1691) | 1638 Lynn, Essex County, Massachusetts | 1691 East Haddam, Middlesex County, Connecticut | Daniel Cone (1626-1706) |
| Hannah Spencer (1641-1691) | |||
| Alice Spencer (1641-1714) | |||
| Marah Spencer (1642-1714) | |||
| Samuel Spencer (1644-1705) | 27 July 1644 East Haddam, Middlesex County, Connecticut | East Haddam, Middlesex County, Connecticut | Hannah Willey (1642-1681) Miriam Moore (1647-1706) Gertrude Bartlisdotter (1655-1748) |
| Elizabeth Spencer (1646-1688) | |||
| Thomas Spencer (1648-1699) | |||
| Timothy Spencer (1652-1704) | |||
| Ruth Spencer (1654-1744) | |||
| William Spencer (1656-1713) | |||
| Nathaniel Spencer (1658-1722) | |||