Four or more generations of descendants of Margaret Tudor (1489-1541) if they are properly linked:
1. Margaret Tudor (1489-1541)
- 2. James Stewart, Duke of Rothesay (1507-1508)
- 2. unnamed daughter Stewart (1508-1508)
- 2. Arthur Stewart, Duke of Rothesay (1509-1510)
- 2. James V of Scotland (1512-1542)
- 3. James Stewart, Duke of Rothesay (1540-1541)
- 3. Arthur Stewart, Duke of Albany (1541-1541)
- 3. Mary of Scotland (1542-1586)
- 4. James I of England (1566-1625)
- 5. Henry Frederick Stuart, Prince Of Wales (1594-1612) ... (more)
- 5. Elizabeth Stuart (1596-1662) ... (more)
- 5. Margaret Stuart (1598-1600) ... (more)
- 5. Charles I of England (1600-1649) ... (more)
- 5. Robert Stuart, Duke of Kintyre (1602-1602) ... (more)
- 5. Mary Stuart (1605-1607) ... (more)
- 5. Sophia Stuart (1606-1606) ... (more)
- 4. James I of England (1566-1625)
- 3. James Stewart, 1st Earl of Moray (c1531-1570)
- 4. Elizabeth Stuart, 2nd Countess of Moray (bef1569-1591)
- 5. James Stuart, 3rd Earl of Moray (c1581-1638) ... (more)
- 5. Elizabeth Stewart (bef1591-bef1609) ... (more)
- 5. Francis Stuart (bef1591-) ... (more)
- 5. Margaret Stuart (bef1591-1639) ... (more)
- 5. Grizel Stuart (bef1611-) ... (more)
- 4. Annabel Stuart (-1572)
- 4. Margaret Stuart (bef1569-1586)
- 4. Elizabeth Stuart, 2nd Countess of Moray (bef1569-1591)
- 3. Adam Stewart (c1536-bef1606)
- 3. Robert Stewart, 1st Earl of Orkney (1533-1593)
- 4. Henry Stewart (-aft1589)
- 4. Patrick Stewart, 2nd Earl of Orkney (c1568-1615)
- 4. John Stewart, 1st Earl of Carrick (-aft1642)
- 4. James Stewart (-)
- 4. Robert Stewart (-)
- 4. Mary Stewart (-1644)
- 5. Elizabeth Gray (1586-) ... (more)
- 5. Andrew Gray, 7th Lord Gray (-1663) ... (more)
- 5. Jean Gray (-1640) ... (more)
- 5. Agnes Gray (-1667) ... (more)
- 4. Katherine Stewart (-)
- 4. Jean Stewart (c1563-)
- 5. Robert Leslie (c1598-c1675) ... (more)
- 5. David Leslie, 1st Lord Newark (c1601-1694) ... (more)
- 5. Margaret Leslie (-c1668) ... (more)
- 4. Elizabeth Stewart (-)
- 2. unnamed daughter Stewart (1512-1512)
- 2. Alexander Stewart, Duke of Ross (1514-1515)
- 2. Margaret Douglas (1515-1578)
- 3. Henry Stuart, Lord Darnley (1545-1567)
- 4. James I of England (1566-1625)
- 5. Henry Frederick Stuart, Prince Of Wales (1594-1612) ... (more)
- 5. Elizabeth Stuart (1596-1662) ... (more)
- 5. Margaret Stuart (1598-1600) ... (more)
- 5. Charles I of England (1600-1649) ... (more)
- 5. Robert Stuart, Duke of Kintyre (1602-1602) ... (more)
- 5. Mary Stuart (1605-1607) ... (more)
- 5. Sophia Stuart (1606-1606) ... (more)
- 4. James I of England (1566-1625)
- 3. Charles Stuart (c1547-1576)
- 3. Henry Stuart, Lord Darnley (1545-1567)
- 2. Dorothea Stewart (1528-c1528)