Jonathan Penfield was born 25 March 1719 in Groton, New London County, Connecticut, United States to John Penfield (1683-1750) and Ann Cornwall (c1688-1779) and died 23 July 1794 East Hampton, Middlesex County, Connecticut, United States of unspecified causes. He married Elizabeth Shepard (1729-1803) 20 December 1750 in Middletown, Middlesex County, Connecticut, United States.
Children
| Name | Birth | Death | Joined with |
| Elizabeth Penfield (1752-1815) | |||
| Prudence Penfield (1754-) | |||
| Anna Penfield (1755-) | |||
| Sarah Penfield (1756-1846) | |||
| Abel Penfield (1758-1852) | |||
| Jonathan Penfield (1760-1770) | |||
| Samuel Penfield (1761-1761) | |||
| Lucia Penfield (1763-1770) | |||
| Zebulon Penfield (1765-1860) | |||
| Hannah Penfield (1767-1784) | |||
| Vienne Penfield (1769-1799) | |||
| Jonathan Penfield (1772-1776) | |||
| Lucy Sage Penfield (1776-) | |||
Siblings
| Name | Birth | Death | Joined with |
| Stephen Penfield (1713-1749) | 27 January 1713 Groton, New London County, Connecticut, United States | 12 September 1749 Chatham, Barnstable County, Massachusetts, United States | Jerusha Brewer |
| Simeon Penfield (1715-1794) | 2 August 1715 Groton, New London County, Connecticut, United States | 25 August 1794 East Hampton, Middlesex County, Connecticut, United States | Mary Bowers (1728-1819) |
| Jonathan Penfield (1719-1794) | 25 March 1719 Groton, New London County, Connecticut, United States | 23 July 1794 East Hampton, Middlesex County, Connecticut, United States | Elizabeth Shepard (1729-1803) |
| David Penfield (1722-1795) | |||
| Silence Penfield (1724-1792) | |||
| Hannah Penfield (1728-1797) | |||
| John Penfield (1731-1797) | |||