Capt. Isaac Matthews, Jr. was born 29 May 1744 in Yarmouth, Barnstable County, Massachusetts, United States to Isaac Matthews (1712-1790) and Sarah Hedge (1709-1761) and died 2 September 1820 New Haven, Addison County, Vermont, United States of unspecified causes. He married Lydia Read (1751-1819) .
Children
| Name | Birth | Death | Joined with |
| Harry Matthews (-1820) | |||
| David Matthews (1770-1777) | |||
| Joseph Matthews (1772-1845) | |||
| Isaac Matthews (1777-1791) | |||
| James Read Matthews (1786-1864) | |||
| Lydia Matthews (1789-1843) | |||
| Sarah Matthews (1790-1875) | |||
| Amos Matthews (1792-1858) | |||
Siblings
| Name | Birth | Death | Joined with |
| Isaac Matthews (1744-1820) | 29 May 1744 Yarmouth, Barnstable County, Massachusetts, United States | 2 September 1820 New Haven, Addison County, Vermont, United States | Lydia Read (1751-1819) |
| Barnabas Matthews (1749-1826) | 4 July 1749 Yarmouth, Barnstable County, Massachusetts, United States | 23 December 1826 Whitehall, Washington County, New York, United States | Desire Rider (1747-1816) |